AffiVET: A Next-Generation PCR Kit for Precise Detection of Toxoplasma gondii in Veterinary Specimens

Toxoplasma gondii is a protozoan parasite that infects a wide range of warm-blooded animals, including humans. It is a significant cause of zoonotic disease, leading to toxoplasmosis, which can result in serious health issues, particularly in immunocompromised individuals and pregnant women. Accurate and rapid detection of T. gondii is essential for effective diagnosis and management of the infection. Polymerase Chain Reaction (PCR) is a widely used molecular technique for the detection of T. gondii due to its high sensitivity and specificity. The AffiVET T. gondii PCR Kit is a commercial product designed for this purpose.

Kit Overview

The AffiVET T. gondii PCR Kit is a ready-to-use system for the qualitative detection of T. gondii DNA in various sample types, including blood, tissue, and cerebrospinal fluid. The kit includes all necessary reagents for PCR amplification, including primers and probes specific to the T. gondii genome, a positive control, and a negative control.


PCR Master Mix

Contains DNA polymerase, dNTPs, MgCl2, and a buffer optimized for PCR.

Primers and Probes

Specific for T. gondii B1 gene, known for its high copy number and stability, ensuring high sensitivity.

Positive Control

T. gondii DNA to verify the PCR amplification.

Negative Control

To check for contamination or non-specific amplification.

Template DNA

Extracted from the sample using an appropriate DNA extraction method.

DNA Extraction

Prior to PCR, DNA is extracted from the samples using a suitable extraction kit or protocol. The quality and quantity of the extracted DNA are critical for the success of the PCR assay.

PCR Amplification


    Add 10 µL of extracted DNA to the PCR tube containing 15 µL of PCR Master Mix.

    Include 5 µL of positive control DNA in a separate tube and 5 µL of nuclease-free water in another tube as the negative control.

      Thermal Cycling Conditions

      Initial denaturation at 95°C for 10 minutes.

      Followed by 40 cycles of:

      • Denaturation at 95°C for 15 seconds.
      • Annealing and extension at 60°C for 1 minute.


      The PCR products are detected via fluorescence, with the probes emitting a signal upon hybridization with the target DNA. Real-time PCR machines quantify the fluorescence intensity, correlating with the amount of T. gondii DNA in the sample.

      Interpretation of Results

      Positive Result

      Presence of T. gondii DNA indicated by the amplification curve of the sample crossing the threshold line, consistent with the positive control.

      Negative Result

      No amplification curve or a curve below the threshold line, consistent with the negative control.

      Invalid Result

      If the negative control shows amplification or the positive control does not, the results are invalid, and the assay should be repeated.

      Performance Characteristics


      The kit can detect as low as 1-10 copies of T. gondii DNA per reaction.


      No cross-reactivity with DNA from other related protozoa or host DNA, ensuring accurate detection of T. gondii.


      Consistent results across different runs, operators, and instruments, ensuring reliability.


      Clinical Diagnostic

      Detection of T. gondii in patients suspected of having toxoplasmosis.

      Veterinary Diagnostics

      Screening of livestock and pets for T. gondii infection.


      Studying the epidemiology and pathogenicity of T. gondii.


      The AffiVET T. gondii PCR Kit is a reliable, sensitive, and specific tool for the detection of T. gondii DNA. Its ease of use and robust performance make it suitable for various applications in clinical, veterinary, and research settings. Accurate detection of T. gondii through this kit can significantly aid in the diagnosis and management of toxoplasmosis, ultimately contributing to better health outcomes.

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