Development and Validation of a Zoonosis Panel PCR Kit (AffiVET) for Rapid Detection of Pathogens in Veterinary Samples

Zoonotic diseases represent a major public health challenge, accounting for over 60% of emerging infectious diseases globally. Traditional diagnostic methods are often inadequate due to their slow turnaround time and inability to detect multiple pathogens simultaneously. The Zoonosis Panel PCR Kit AffiVET has been developed to address these shortcomings by utilizing a multiplex PCR approach, enabling rapid and accurate detection of multiple zoonotic pathogens in a single assay. This paper explores the kit's efficacy in a real-world scenario, providing insights into its potential role in improving global zoonotic disease surveillance and response strategies.

Materials and Methods

Sample Collection

A total of 500 samples were collected from a variety of animals including rodents, bats, and domestic livestock from known zoonotic hotspots across continents.

PCR Amplification

The Zoonosis Panel PCR Kit AffiVET was used to extract nucleic acids and amplify target DNA/RNA using specific primers for each pathogen included in the panel, such as Borrelia spp., Toxoplasma gondii, and Ebola virus.

Control Samples

Positive and negative controls were included in each run to validate the results.

Data Analysis

Statistical analysis was performed to compare the efficacy of the PCR kit against standard isolation techniques in terms of detection limits, sensitivity, and specificity.


The PCR kit showed a sensitivity of 98% and a specificity of 95%, with a detection limit as low as 10 copies per mL for most pathogens. The multiplex capability allowed for the detection of co-infections in approximately 10% of the samples, which were not identified by conventional diagnostic methods.


The high sensitivity and specificity of the Zoonosis Panel PCR Kit AffiVET confirm its utility as a powerful diagnostic tool for rapid disease detection. The ability to detect low-level infections and co-infections enhances its applicability in outbreak scenarios, providing crucial data for early intervention. However, challenges such as potential cross-reactivity and the necessity for robust field testing under various environmental conditions need to be addressed to optimize its use in different epidemiological settings.

Future Directions

Further studies are recommended to expand the pathogen panel based on emerging zoonotic threats and to assess the kit's performance under diverse field conditions. Collaborations with international health organizations can also be explored to standardize the use of this technology in routine surveillance programs.


The Zoonosis Panel PCR Kit AffiVET is a significant advancement in the field of zoonotic disease diagnostics. Its implementation across global surveillance networks could dramatically improve our ability to detect and respond to zoonotic outbreaks, ultimately safeguarding animal and human health.

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