Utilization of Diagnostic Panels in Feline Medicine: An Overview of Applications and Efficacy

Panel Cats AFFIVET is a diagnostic tool used in veterinary medicine for the detection of various feline infectious diseases. This article aims to provide a detailed and technical overview of the Panel Cats AFFIVET, including its composition, methodology, applications, and interpretation of results.

Composition and Methodology


Panel Cats AFFIVET typically consists of a series of specific antigens and antibodies designed to detect the presence of various pathogens in feline blood samples. The panel may include tests for:

  • Feline Immunodeficiency Virus (FIV)
  • Feline Leukemia Virus (FeLV)
  • Feline Coronavirus (FCoV)
  • Feline Calicivirus (FCV)
  • Feline Herpesvirus (FHV)


The methodology of Panel Cats AFFIVET involves several steps:

  • Sample Collection: A blood sample is collected from the cat using standard veterinary procedures.
  • Sample Preparation: The blood sample is prepared, typically by centrifugation to separate the serum or plasma.
  • Application of Sample: The prepared sample is applied to the test device, which contains immobilized antigens or antibodies specific to the pathogens being tested.
  • Incubation: The test device is incubated for a specified period to allow for the interaction between the sample and the test reagents.
  • Detection: The presence of specific antigens or antibodies in the sample is detected through colorimetric changes, often utilizing enzyme-linked immunosorbent assay (ELISA) technology.


Diagnostic Use

Panel Cats AFFIVET is primarily used for the diagnosis of infectious diseases in cats. The panel provides veterinarians with a quick and reliable means of identifying multiple pathogens from a single sample. This is particularly useful in clinical settings where rapid diagnosis can significantly influence treatment decisions and outcomes.

Screening and Monitoring

In addition to diagnosis, Panel Cats AFFIVET can be used for screening purposes, especially in multi-cat environments such as shelters and catteries. Regular screening helps in the early detection and management of infectious diseases, thereby reducing the spread of pathogens. Monitoring of disease progression and response to treatment is another critical application, providing insights into the efficacy of therapeutic interventions.

Interpretation of Results

Positive Results

A positive result indicates the presence of specific antigens or antibodies in the cat's blood sample, suggesting an active or past infection. It is essential to consider the clinical signs and history of the animal in conjunction with the test results to make an accurate diagnosis.

Negative Results

A negative result suggests the absence of detectable levels of specific antigens or antibodies in the sample. However, false negatives can occur, especially if the sample is collected during the early stages of infection or if there are issues with sample handling or test performance.

Sensitivity and Specificity

The sensitivity and specificity of Panel Cats AFFIVET are crucial parameters that determine its diagnostic accuracy. Sensitivity refers to the test's ability to correctly identify infected animals, while specificity refers to the test's ability to correctly identify non-infected animals. High sensitivity and specificity are desired to minimize false positives and false negatives.


While Panel Cats AFFIVET is a powerful diagnostic tool, it has limitations:

  • Cross-reactivity: Some tests may exhibit cross-reactivity with non-target pathogens, leading to false-positive results.
  • Sample Quality: Poor sample quality can affect the accuracy of the test results.
  • Stage of Infection: The timing of sample collection relative to the stage of infection can influence the test outcome.

Panel Cats AFFIVET is a vital tool in veterinary diagnostics, offering a comprehensive approach to detecting multiple feline infectious diseases. Understanding its composition, methodology, applications, and limitations is essential for its effective use in clinical practice. Accurate interpretation of results, coupled with clinical correlation, ensures optimal patient management and disease control in feline populations.

Application of the AffiVET PCR Kit for Rapid Detection of Contagious Bovine Pleuropneumonia (CBPP)